Lenti-ONE VEGF165b carries the VEGF165b cDNA. It encodes the vascular endothelial growth factor 165b (sequence from human genome, AF430806.1, 606 pb). This protein is an antagonist of VEGF165a and inhibits angiogenesis process. It may be used in anti-angiogenesis experimental gene transfer assays.
VEGF165b SIGNALING PATHWAY IN ENDOTHELIAL CELLS
Examples of Applications
- Use with flt1 promoter to target the expression in endothelial cells
- Use in vitro or in vivo to inhibit angiogenesis
- Use in vitro, in vivo to study cancer mechanisms
VEGF EXPRESSION IN VITRO USING NON-INTEGRATIVE LENTI-ONE
Transduction of 293T cells in vitro with non-integrative Lenti-ONE VEGF121, VEGF165 or VEGF165b (100ng p24) induces transgene expression and VEGF secretion in cell culture medium (measured by ELISA).
Results provided by GEG Tech R&D