Lenti-ONE tRFP-2A-Luc2 carries the expression sequences of a fusion protein containing:
- tRFP cDNA which encodes turbo red fluorescent protein (artificial sequence derived from sea anemone Entacmaea quadricolor, KT358727.1, 693 pb). tRFP is a fluorescent reporter protein allowing the monitoring of expression and transduction by fluorescent microscopy or by flow cytometry
- Sequence 2A, a cleavage sequence (from Porcine teschovirus 11, GenBank: ACT66686.1)
- Luc2 cDNA which encodes the firefly luciferase 2 (artificial sequence derived from Photinus pyralis genome, AB644228.1, 1647 pb). Luc2 is a reporter enzyme allowing monitoring expression and transduction by bioluminescence assay.
Examples of Applications
- Use in vitro, in vivo as a control with another vector