Lenti-ONE Cre-NLS

Description

Cre-NLS cDNA encodes a cyclization recombinase fused with a nuclear localization signal (sequence from Enterobacterio phage P1 genome, AB449974.1/AX343218.1, 1053 pb). This tyrosine recombinase recognizes LoxP sequences and mediates recombination between two DNA fragments. This reaction can be used to excise a DNA fragment flanked by 2 loxP sequences or to integrate a DNA fragment into a single loxP sequence. Addition of a nuclear localization signal increases the efficiency of the enzyme.

Examples of Applications

For this lentiviral vector, we recommend choosing a non-integrative mutant (IN(D64V) for example). This will make the Cre expression transient so that the potential toxicity of the recombinase is not deleterious.

• Use in vitro, in vivo or for transgenesis purposes to mediate site specific integration of a substrate DNA vector which contains a LoxP site into a target LoxP site
• Use in vitro, in vivo or for transgenesis purposes to mediate excision of a DNA sequence flanked by LoxP sites, eg: to generate a conditional KO or to induce expression of a specific transgene
• Use in vitro, in vivo or for transgenesis purposes for Recombinase-Mediated Cassette Exchange in cells containing a target sequence flanked by LoxP sites

If you wish to order a lentiviral vector with loxP sites, and the transgene that you need to integrate, please contact us and we will gladly produce it for you.

Results

EXPRESSION OF CRE RECOMBINASES BY LENTI-ONE TRANS VECTORS IN CV1-5B CELLS MEDIATES EXCISION OF A TARGET SEQUENCE