Cas9 cDNA encodes for protein-9 nuclease from Streptococcus pyogenes. (Sequence, WP_010922251.1, 4128bp). Coexpression of Cas9 with an artificial single guide RNA (sgRNA) which fuses the crRNA with the tracrRNA offers the possibility to target and modify DNA sequences of interest.
Examples of Applications
- Use for CRISPR-Cas9 genome editing technology
- Use with Lenti-ONE gRNA for a two-vector CRISPR System