Lenti-ONE GFP carries the transgene green fluorescent protein. Its expression can be assessed by fluorescent microscopy or by flow cytometry, thus giving valuable informations about the transduction efficiency of the vector.
Lenti-ONE Cas9 contains the cDNA encoding for protein-9 nuclease from Streptococcus pyogenes. (Sequence, WP_010922251.1, 4128bp). Coexpression of Cas9 with an artificial single guide RNA (sgRNA) which fuses the crRNA with the tracrRNA offers the possibility to target and modify DNA sequences of interest.
Lenti-ONE vectors provide an efficient method to drive the suitable level of Cas9 expression. Lenti-ONE Cas9 is available at very high titer >1E9TU/mL, with several packaging options. Thanks to multiple vector configurations, you can build it with a wide range of options, such as:
Lenti-ONE Cas9 can be used alone or co-transduced with other Lenti-ONE vectors.