Menu
GDNF cDNA encodes the glial cell derived neurotrophic factor (sequence from human genome, BC128108.1, 636 pb). This neurotrophic factor may be used to promote neuronal survival in experimental gene therapy assays.
Igniting various cascades, GDNF can promote the transcription of survival factors through a Ras/MAPK/CREB pathway, and/or inhibit some death factors thanks to the P13K/AKT pathway.
When pseudotyped with a Mokola envelope, Lenti-ONE GDNF limits transduction to glial cells. It can be useful to overexpress GDNF in glial cells around damaged neurons.
INFLUENCE OF PSEUDOTYPING ON THE TROPISM OF LENTI-ONE VECTORS
Results provided by GEG Tech R&D
GFP expression in murine and primate brain after stereotactic injection of Lenti-ONE PGK GFP. GFAP labeling shows glial cells, NeuN labeling shows neurons.
GDNF EXPRESSION IN VITRO USING LENTI-ONE IN SCHWANN CELLS
Results provided by GEG Tech R&D
Transduction of primary Schwann cells by Lenti-ONE GDNF, or non-integrative Lenti-ONE GDNF. GDNF is secreted by cells and accumulates in cell culture medium and can be measured by ELISA.
