Lenti-ONE PhiC31-NLS

APPLICATIONS

  • Angiogenesis
  • Apoptosis, Oxidative Stress
  • CAR T-cells
  • CRISPR
  • Differentiation
  • Dominant Negative Mutants
  • Neurology, Ophthalmology
  • Optogenetics
  • Recombinases, Editors
  • Reporters and selection
  • RNA Interference

TRANSGENES

Lenti-ONE PhiC31-NLS

Lenti-ONE GFP carries the transgene green fluorescent protein. Its expression can be assessed by fluorescent microscopy or by flow cytometry, thus giving valuable informations about the transduction efficiency of the vector.

Lenti-ONE PhiC31-NLS

DESCRIPTION

PhiC31-NLS cDNA encodes a PhiC31 phage recombinase fused with a Nuclear Localization Signal (sequence from Streptomyces phage PhiC31 genome, AJ006589.2, 1836 pb). This serine recombinase recognizes an attB (or pseudo attB) sequence and an attP (or pseudo-attP) sequence and mediates recombination between both sequences. This allows, for instance, the integration of a DNA fragment of interest into host cell chromosomes.

RECOMBINATION MEDIATED BY PHIC31 INTEGRASE

Lenti-ONE PhiC31-NLS -

Two non-integrative Lenti-ONE® vectors are used to mediate integration:

  • the first vector is a non-integrative Lenti-ONE PhiC31-NLS. It delivers the phiC31 recombinase

  • the second vector contains the sequence of interest with the attB sequence needed for the recombination with pseudo attP sites present in most mamalian genomes.

If you wish to order a lentiviral vector with attB sequences and your tansgene of interest, please contact us.

EXAMPLES OF APPLICATIONS

  • Use in vitro, in vivo or for transgenesis purposes to mediate site specific integration of a substrate DNA vector which contains an attB site into attP or pseudo attP sites

  • Use in vitro, in vivo or in transgenesis purpose for Recombinase-Mediated Cassette Exchange in cells already containing a sequence flanked by attP or attB sites

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