Lenti-ONE GFP carries the transgene green fluorescent protein. Its expression can be assessed by fluorescent microscopy or by flow cytometry, thus giving valuable informations about the transduction efficiency of the vector.
PhiC31-NLS cDNA encodes a PhiC31 phage recombinase fused with a Nuclear Localization Signal (sequence from Streptomyces phage PhiC31 genome, AJ006589.2, 1836 pb). This serine recombinase recognizes an attB (or pseudo attB) sequence and an attP (or pseudo-attP) sequence and mediates recombination between both sequences. This allows, for instance, the integration of a DNA fragment of interest into host cell chromosomes.
RECOMBINATION MEDIATED BY PHIC31 INTEGRASE
Two non-integrative Lenti-ONE® vectors are used to mediate integration:
If you wish to order a lentiviral vector with attB sequences and your tansgene of interest, please contact us.