BDNF cDNA encodes the brain-derived neurotrophic factor isoform a (sequence from human genome, NM_001143805.1/CCDS7866.1, 744 pb). This neurotrophic factor may be used to promote neuronal survival in experimental gene therapy assays.
Igniting various cascades, BDNF can promote the transcription of survival factors through a Ras/MAPK/CREB pathway, and/or inhibit some death factors thanks to the P13K/AKT pathway.
Examples of Applications
- Use in vitro or in vivo to study nervous system
- Use in vitro or in vivo to study neurodegenerescence pathways and develop experimental therapy for neurodegenerative diseases
When pseudotyped with a Mokola envelope, Lenti-ONE BDNF limits transduction to glial cells. It can be useful to overexpress BDNF in glial cells around damaged neurons.
INFLUENCE OF PSEUDOTYPING ON THE TROPISM OF LENTI-ONE VECTORS
GFP expression in murine and primate brain after stereotactic injection of Lenti-ONE PGK GFP. GFAP labeling shows glial cells, NeuN labeling shows neurons.