lenti-cas9-gfp

Lenti-ONE Cas9-GFP

From €330.00

Description

Cas9 cDNA encodes for protein-9 nuclease from Streptococcus pyogenes. (Sequence, WP_010922251.1, 4128bp). Coexpression of Cas9 with an artificial single guide RNA (sgRNA) which fuses the crRNA with the tracrRNA offers the possibility to target and modify DNA sequences of interest.

CRISPR-Cas9

In this vector, Cas9 is fused with GFP cDNA encoding a green fluorescent protein (sequence from Aequorea victoria genome, AAB02574.1, 717 pb). This fluorescent reporter protein allows monitoring expression and transduction by fluorescent microscopy or by flow cytometry.

Examples of Applications

  • Use for CRISPR-Cas9 genome editing technology
  • Use with Lenti-ONE gRNA for a two-vector CRISPR System