Cas9 cDNA encodes for protein-9 nuclease from Streptococcus pyogenes. (Sequence, WP_010922251.1, 4128bp). Coexpression of Cas9 with an artificial single guide RNA (sgRNA) which fuses the crRNA with the tracrRNA offers the possibility to target and modify DNA sequences of interest.
In this vector, Cas9 is fused with GFP cDNA encoding a green fluorescent protein (sequence from Aequorea victoria genome, AAB02574.1, 717 pb). This fluorescent reporter protein allows monitoring expression and transduction by fluorescent microscopy or by flow cytometry.
Examples of Applications
- Use for CRISPR-Cas9 genome editing technology
- Use with Lenti-ONE gRNA for a two-vector CRISPR System