Flpo cDNA is a codon-optimized version of Flp (flippase) which encodes a tyrosine recombinase (sequence from Saccharomyces cerevisiae genome, AKA10112.1, 1299 pb). This recombinase recognizes FRT sequences and mediates recombination between two FRT sites. This reaction can be used to excise a DNA fragment flanked by FRT sequences or to integrate a DNA fragment into a single FRT sequence.
The sequence of interest and Flpo are delivered by two non-integrative Lenti-ONE vectors.
Flpo is delivered by a non-integrative Lenti-ONE vector.
Examples of Applications
For this lentiviral vector, we recommend choosing a non-integrative mutant (IN(D64V) for example). This will make Flpo expression transient so that the potential toxicity of the recombinase is not deleterious.
- Use in vitro, in vivo or for transgenesis purposes to mediate site specific integration of a substrate DNA vector which contains a FRT site into a target FRT site
- Use in vitro, in vivo or for transgenesis purposes to mediate excision of a DNA sequence flanked by FRT sites, eg: to generate a conditional KO or to induce expression of a specific transgene
- Use in vitro, in vivo or for transgenesis purposes for Recombinase-Mediated Cassette Exchange in cells containing a target sequence flanked by FRT sites