- Use for CRISPR-Cas9 genome editing technology
Lenti-ONE GFP carries the transgene green fluorescent protein. Its expression can be assessed by fluorescent microscopy or by flow cytometry, thus giving valuable informations about the transduction efficiency of the vector.
Using the CRISPR-Cas9 system, you can target and modify DNA sequences of interest. This All-in-One system contains both the Cas9 nuclease sequence, and the custom gRNA:
This system allows you to simplify the transduction step by only using one vector. Plus, it makes sure that Cas9 and the gRNA are both expressed in the target cell.