- Use for CRISPR-Cas9 genome editing technology
Lenti-ONE Cas9-gRNA
APPLICATIONS
- Angiogenesis
- Apoptosis, Oxidative Stress
- CAR T-cells
- CRISPR
- Differentiation
- Dominant Negative Mutants
- Neurology, Ophthalmology
- Optogenetics
- Recombinases, Editors
- Reporters and selection
- RNA Interference
TRANSGENES
Lenti-ONE Cas9-gRNA
Lenti-ONE GFP carries the transgene green fluorescent protein. Its expression can be assessed by fluorescent microscopy or by flow cytometry, thus giving valuable informations about the transduction efficiency of the vector.
DESCRIPTION
Using the CRISPR-Cas9 system, you can target and modify DNA sequences of interest. This All-in-One system contains both the Cas9 nuclease sequence, and the custom gRNA:
- Cas9 cDNA encodes for CRISPR associated protein-9 nuclease from Streptococcus pyogenes. (Sequence, WP_010922251.1, 4128bp).
- the gRNA that will target your sequence of interest, under the control of U6 promoter.
This system allows you to simplify the transduction step by only using one vector. Plus, it makes sure that Cas9 and the gRNA are both expressed in the target cell.
