Using the CRISPR-Cas9 system, you can target and modify DNA sequences of interest. This All-in-One system contains both the Cas9 nuclease sequence, and the custom gRNA:
- Cas9 cDNA encodes for CRISPR associated protein-9 nuclease from Streptococcus pyogenes. (Sequence, WP_010922251.1, 4128bp).
- the gRNA that will target your sequence of interest, under the control of U6 promoter.
This system allows you to simplify the transduction step by only using one vector. Plus, it makes sure that Cas9 and the gRNA are both expressed in the target cell.
Examples of Applications
- Use for CRISPR-Cas9 genome editing technology