Neo cDNA encodes the neomycin phosphotransferase (sequence from Escherichia coli genome, DD224401.1, 795 pb). This enzyme is responsible for inactivation of aminoglycoside antibiotics (e.g. kanamycin, neomycin, geneticin). It is used as a selection marker and allows, for instance, to create genetically engineered cell lines for in vitro assays.
Examples of Applications
- Use in vitro, in vivo as a control with another vector