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Episomal lentiviral vectors confer erythropoietin expression in dividing cells Lentiviral vectors have evolved over the last decade as powerful and reliable gene transfer tools for dividing and non-dividing cells because they possess a large packaging capacity, weak immunogenesis, and a high flexible of design. However, in some cases, the integrating…
Genetic Engineering: Evolution and Revolution Over the last years, new genetic engineering technologies have emerged and undoubtedly show up game-changing. Genome engineering is since a long time explored and used because there is a lot at stake here in many fields. Indeed, the possibility to add, remove or change precisely…
Sendai virus, an RNA virus with no risk of genomic integration, delivers CRISPR/Cas9 for efficient gene editing A significant recent advance in genome engineering is the development of the CRISPR/Cas9 system for nuclease based genome editing. However, several cell types are not easily transfected and in vivo delivery of the…
Lentiviral expression system for the purification of secreted proteins from human cell cultures Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. CHO cells are mostly used but recent studies highlight that proteins produced in this cell line have a different…
Pseudotyping of lentiviral vector with novel vesiculovirus envelope glycoproteins derived from Chandipura and Piry viruses Lentiviral vectors are commonly pseudotyped with the heterologous envelope VSV-G due to its broad host tropism and stability conferred. However, in some cases, the sensibility of VSV-G by human complement hinders the use of lentiviral…
Lentiviral vectors: the secret behind the rise of CAR-T therapies CAR-T has become a hype within the biotech field recently. Companies promising to deliver this new generation of therapies are shining on stock markets, sometimes leading to billion-dollar valuations. But the rise of CAR-Ts would not have been possible without…
Surface modification via strain-promoted click reaction facilitates targeted lentiviral transduction Since the last decade, the Lentiviral Vector has emerged as a promising vector for gene delivery. This is coming from its high efficiency to transduce a large variety of cells in vitro as well as in vivo. Moreover, the Lentiviral Vector has a…
Kinetics of lentiviral vector transduction in human CD34+ cells Human hematopoietic stem cells (HSCs) are refractory to the lentiviral vector transduction. Improvement of transduction efficiency for human HSCs remains crucial for further development of gene therapy especially for non-immunodeficiency diseases. In this report, the authors investigated which step(s) could be…
Development of 3rd Generation Cocal Envelope Producer Cell Lines for Robust Lentiviral Gene Transfer into Hematopoietic Stem Cells and T Cells Lentiviral vectors (LVs) are currently considered the gold standard for hematopoietic stem cell (HSC) gene therapy and for immunotherapies with genetically modified T cells. LVs have commonly been pseudotyped…
Lentivirus pre-packed with Cas9 protein for safer gene editing The CRISPR/Cas9 system has democratized genome editing and opens the door to an unprecedented use of genome editors. This powerful method is allowing researchers to ask questions that were previously unaskable, leading to new insights into the basis of fundamental biological…