Preclinical Evaluation of a Lentiviral Vector for Huntingtin Silencing Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder resulting from a polyglutamine expansion in the huntingtin (HTT) protein. There is currently no cure for this disease, but recent studies suggest that RNAi to downregulate the expression of both normal and…
Integrase-Deficient Lentiviral Vector as an All-in-One Platform for Highly Efficient CRISPR/Cas9-Mediated Gene Editing The CRISPR/Cas9 systems have revolutionized the field of genome editing by providing unprecedented control over gene sequences and gene expression in many species, including humans. Lentiviral vectors (LVs) are an important means of delivering CRISPR/Cas9 components due…
Preclinical and Personalized Breast Cancer Research As scientists involved in life science research, we would like to let you know about the research project on breast cancer, led by the Brisken Lab in EPFL, Switzerland. The scientific team of GEG Tech is proud to be a part of it, designing…
Episomal lentiviral vectors confer erythropoietin expression in dividing cells Lentiviral vectors have evolved over the last decade as powerful and reliable gene transfer tools for dividing and non-dividing cells because they possess a large packaging capacity, weak immunogenesis, and a high flexible of design. However, in some cases, the integrating…
Genetic Engineering: Evolution and Revolution Over the last years, new genetic engineering technologies have emerged and undoubtedly show up game-changing. Genome engineering is since a long time explored and used because there is a lot at stake here in many fields. Indeed, the possibility to add, remove or change precisely…
Sendai virus, an RNA virus with no risk of genomic integration, delivers CRISPR/Cas9 for efficient gene editing A significant recent advance in genome engineering is the development of the CRISPR/Cas9 system for nuclease based genome editing. However, several cell types are not easily transfected and in vivo delivery of the…
Lentiviral expression system for the purification of secreted proteins from human cell cultures Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. CHO cells are mostly used but recent studies highlight that proteins produced in this cell line have a different…
Pseudotyping of lentiviral vector with novel vesiculovirus envelope glycoproteins derived from Chandipura and Piry viruses Lentiviral vectors are commonly pseudotyped with the heterologous envelope VSV-G due to its broad host tropism and stability conferred. However, in some cases, the sensibility of VSV-G by human complement hinders the use of lentiviral…
Lentiviral vectors: the secret behind the rise of CAR-T therapies CAR-T has become a hype within the biotech field recently. Companies promising to deliver this new generation of therapies are shining on stock markets, sometimes leading to billion-dollar valuations. But the rise of CAR-Ts would not have been possible without…
Surface modification via strain-promoted click reaction facilitates targeted lentiviral transduction Since the last decade, the Lentiviral Vector has emerged as a promising vector for gene delivery. This is coming from its high efficiency to transduce a large variety of cells in vitro as well as in vivo. Moreover, the Lentiviral Vector has a…
