Miniaturized CRISPR protein opens door for gene therapy with adeno-associated virus
The commonly used adeno-associated virus is small, and although some Cas9 proteins can enter it, Cas12a proteins are generally too large. However, researchers have identified a relatively small version of Cas12a, called EbCas12a, which occurs naturally in a species of the bacterial class Erysipelotrichia. They increased the gene-editing efficiency by deliberately replacing one of the […]