CRISPR/Cas9 deletions induce adverse on-target genomic effects

CRISPR/Cas9 deletions induce adverse on-target genomic effects - Blog

In a recent study, a team of researchers used a CRISPR/Cas9 system to evaluate the use of tRNA by deleting two tRNA genes from the genomes of hyperhepatocellular carcinoma and human chronic myeloid quasi-haploid leukaemia cells. The authors discovered numerous unexpected genomic changes at the target region using an improved droplet-based target enrichment approach followed […]