Erythropoietin expression in dividing cells

Episomal lentiviral vectors confer erythropoietin expression in dividing cells

Lentiviral vectors have evolved over the last decade as powerful and reliable gene transfer tools for dividing and non-dividing cells because they possess a large packaging capacity, weak immunogenesis, and a high flexible of design. However, in some cases, the integrating feature of lentiviral vectors may cause unbalanced gene expression, gene silencing and insertional mutagenesis. The sMARt design of non-integrating lentiviral vectors (NILV) can avoid these problems because they do not integrate into the cell genome.

In the related study, the scientists incorporated a tetramer of a 155-bp module from human β-interferon S/MAR sequences into non-integrating lentiviral vectors. This tetramer sequence can function as an origin of replication and maintain the episomal replication of the NILV in dividing cells. They tested an S/MAR-based NILV to see whether it can maintain a therapeutic gene expression in dividing cells. They observed that S/MAR can retain episomal expression through successive rounds of cell division up to 74 days.

GEG Tech offers a wide range of lentiviral vectors including Lenti-ONE EPI (ie NILV) which induce transient expression in dividing cells and stable expression in non-dividing cells. GEG Tech has observed more than one year of in vivo expression after injection in the retina of mice with Lenti-ONE EPI.

Furthermore, GEG Tech provides several types of Lenti-ONE EPI which have different features of expression to adjusting your project.

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