Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles
RNA delivery is an attractive strategy to achieve transient gene expression in research projects and in cell- or gene-based therapies. However, RNA transfer tools developed until that are not enough efficient to be used in primary cells and in vivo. Viral vector constitute an efficient platform for nucleic acid delivery. In this study, the authors […]
DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs
Recently, Estel Aparicio-Prat et al. published a study about a new genome editing system based on CRISPR and lentiviral vector technologies, this tool was specifically designed to knockout long non-coding RNAs. They spell this new system DECKO for Double Excision CRISPR Knockout. The key feature of DECKO is the used of e lentiviral vectors expressing […]
