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LENTIVIRAL VECTORS: MULTIFACETED TOOLS

 

Lentiviral vectors are very efficient tools for delivery DNA sequences of interest in vitro and in vivo. Their high titer production and their high flexibility of pseudotyping make Lentiviral vectors a tool of choice in gene transfer. In their original form, they allow stable integration of their genome in the target cell chromosomes. The transgene expression they mediate is thus maintained in dividing cells. GEG-tech Lenti‑ONE™ products meet these features. Furthermore, GEG Tech proposed two lines of products, issued from GEG Tech R&D team innovations: Lenti‑ONE™ Epi and Trans. Lenti‑ONE™ Epi and Lenti‑ONE™ Trans result from the modification of some features of lentiviral vectors, thus enlarging the scope of applications of these tools.

Lenti‑ONE™ Epi are Integration-Deficient lentiviral vectors. They contain mutations of the viral integrase, leading to the generation of episomal vector genomes. Lenti‑ONE™ Epi can be used for transient expression in dividing cells or long term expression in non-dividing cells without the limits inherent to the integration of the vector.

Lenti‑ONE™ Trans are Reverse transcription-Deficient lentiviral vectors. They result from the mutation of the reverse-transcriptase, which leads to the production of a vector whose genome cannot be reverse-transcribed. Consequently, there is no DNA synthesis and the RNA vector genome is taken in charge by the transduced cell machinery like a mRNA. Lenti‑ONE™ Trans thus allow a fully transient expression regardless the type of cell (dividing or not).

description of the 3 types of vectors

Lenti‑ONE™: Reverse Transcription and integration steps are completed leading to integration into host cell chromatin of a double stranded DNA lentiviral genome.

Lenti‑ONE™ Epi: The integration step is blocked so that the double stranded DNA lentiviral genome resulting from Reverse Transcription remains episomal.

Lenti‑ONE™ Trans: Reverse Transcription is blocked so that the single stranded RNA lentiviral genome delivered by the particle is taken over by cell machinery as a mRNA and is directly translated in protein.

 

 

 

 

SCOPE OF APPLICATIONS

Comparison of application possibilities offered by mostly used gene transfer methods

  lentiviral vectors have many applications

 

Lenti-ONE™

Lenti-ONE™ Epi

Lenti-ONE™ Trans