LENTIVIRAL VECTORS: MULTIFACETED TOOLS
Lentiviral vectors are very efficient tools for delivery DNA sequences of interest in vitro and in vivo. Their high titer production and their high flexibility of pseudotyping make Lentiviral vectors a tool of choice in gene transfer. Furthermore, GEG Tech offers multiple mutants, issued from GEG Tech R&D team innovations. The modification of some features of lentiviral vectors enlarges the scope of applications of these tools.
- Learn more about the kinetics of expression
Integrating Lentiviral Vectors
In their original form, they allow stable integration of their genome in the target cell chromosomes. The transgene expression they mediate is thus maintained in dividing cells.
- in vitro and in vivo long term expression in dividing cell and in non-dividing cells
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- Protocol for Transduction with integrating LV
- Generation of a stable cell line
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- Protocol for Generation of clonal cell lines
- Transgenesis
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- Learn more about Transgenesis using lentiviral vectors
Integrase-Deficient Lentiviral Vectors
Integration-Deficient lentiviral vectors (mutations D64V, N and LQ) lead to the generation of episomal vector genomes. They can be used for transient expression in dividing cells or long term expression in non-dividing cells without the limits inherent to the integration of the vector.
- in vitro and in vivo long term expression in non-dividing cells or transient expression in dividing cells
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- Protocol for Transduction with IDLV
- Genome editing tools delivery for in vitro and in vivo applications and transgenesis (KO/KI)
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- Protocol for Generate KO with ZFNs
- IPSCs Generation
Reverse Transcriptase-Deficient Lentiviral Vectors
Reverse transcription-Deficient lentiviral vectors result from the mutation of the reverse-transcriptase (mutations D110E, E478Q and D110E/E478Q), which leads to the production of a vector whose genome cannot be reverse-transcribed. Consequently, there is no DNA synthesis and the RNA vector genome is taken in charge by the transduced cell machinery like a mRNA. Those vectors allow a fully transient expression regardless of the type of cells (dividing or not).
- Transient expression of therapeutic factors
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- Protocol for Transduction with RTDLV
- Genome editing tools delivery for in vitro and in vivo applications and transgenesis (KO/KI)
SCOPE OF APPLICATIONS
Comparison of application possibilities offered by mostly used gene transfer methods