The Self-Inactivating KamiCas9 System for the Editing of CNS Disease Genes
In this article, Merienne et al. designed a smart lentiviral vector system to improve the safety for genome editing of CNS disease gene. They characterized their CRISPR self-inactivating system (KamiCas9) for permanent disruption of huntingtin gene in the context of Huntington’s disease (HD). The potency of LV-KamiCas9 was demonstrated in mouse primary neuronal/glial cultures, in […]
Highly Efficient CRISPR/Cas9-Mediated Gene Editing
Integrase-Deficient Lentiviral Vector as an All-in-One Platform for Highly Efficient CRISPR/Cas9-Mediated Gene Editing The CRISPR/Cas9 systems have revolutionized the field of genome editing by providing unprecedented control over gene sequences and gene expression in many species, including humans. Lentiviral vectors (LVs) are an important means of delivering CRISPR/Cas9 components due to their ability to accommodate […]
Genetic Engineering: Evolution and Revolution
Over the last years, new genetic engineering technologies have emerged and undoubtedly show up game-changing. Genome engineering is since a long time explored and used because there is a lot at stake here in many fields. Indeed, the possibility to add, remove or change precisely DNA sequences in many cell lines or organisms have great […]
Sendai virus for CRISPR/Cas9 delivery
Sendai virus, an RNA virus with no risk of genomic integration, delivers CRISPR/Cas9 for efficient gene editing A significant recent advance in genome engineering is the development of the CRISPR/Cas9 system for nuclease based genome editing. However, several cell types are not easily transfected and in vivo delivery of the CRISPR system remains challenging. To […]
Safer gene editing
Lentivirus pre-packed with Cas9 protein for safer gene editing The CRISPR/Cas9 system has democratized genome editing and opens the door to an unprecedented use of genome editors. This powerful method is allowing researchers to ask questions that were previously unaskable, leading to new insights into the basis of fundamental biological processes and to new innovative […]
DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs
Recently, Estel Aparicio-Prat et al. published a study about a new genome editing system based on CRISPR and lentiviral vector technologies, this tool was specifically designed to knockout long non-coding RNAs. They spell this new system DECKO for Double Excision CRISPR Knockout. The key feature of DECKO is the used of e lentiviral vectors expressing […]
