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RNA VECTORS - ON-DEMAND REQUEST FORM

This form will allow to build your RNA lentiviral vector from scratch. You can choose your transgene, promoter, reverse-transcriptase mutation and envelope. The packaging is also customizable, with titers and volume options.

If your needs are not defined yet, you do not have to fill all the options. There are no required fields. Once the form is submitted, our team will guide you to complete the design of your vector.

 

STEP 1: Describe your vector genome


Choose your transgene:

GFP
hrGFPII
Luciferase
Cre-NLS
Neomycin
phiC31-NLS
GDNF
AOX
VEGF165
VEGF165b
VEGF121
MyoD

Other : 

 

Choose your option(s)

Add a post transcriptional regulatory element

Altered for integration (LTR modifications)

Lox sites for Cre-mediated recombinaison (1)

AttB sites for PhiC31-mediated recombinaison (1)

(1) for Cre or PhiC31 mediated recombination, we recommend choosing a non-integrative vector

 

STEP 2: Choose your Reverse-Transcriptase mutation


D110E mutant

E478Q mutant

D110E/E478Q mutant

All three mutations disable the vector reverse-transcriptase. Once in the host cell, the RNA vector genome is used as template for the traduction. The transgene expression will be transient in all cell types.

 

STEP 3: Choose your envelope


VSV glycoprotein
Mokola glycoprotein
Other : 

 

STEP 4: Choose your packaging


Titer:

1E7 TU/mL
1E8 TU/mL

Volume:

50µL
100µL
200µL

 

Comments:


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