Episomal lentiviral vectors confer erythropoietin expression in dividing cells

Lentiviral vectors have evolved over the last decade as powerful and reliable gene transfer tools for dividing and non-dividing cells because they possess a large packaging capacity, weak immunogenesis, and a high flexible of design. However, in some cases, the integrating feature of lentiviral vectors may cause unbalanced gene expression, gene silencing and insertional mutagenesis. The sMARt design of non-integrating lentiviral vectors (NILV) can avoid these problems because they do not integrate into the cell genome.

In the related study, the scientists incorporated a tetramer of a 155-bp module from human β-interferon S/MAR sequences into non-integrating lentiviral vectors. This tetramer sequence can function as an origin of replication and maintain the episomal replication of the NILV in dividing cells. They tested an S/MAR-based NILV to see whether it can maintain a therapeutic gene expression in dividing cells. They observed that S/MAR can retain episomal expression through successive rounds of cell division up to 74 days.

GEG Tech offers a wide range of lentiviral vectors including Lenti-ONE EPI (ie NILV) which induce transient expression in dividing cells and stable expression in non-dividing cells. GEG Tech has observed more than one year of in vivo expression after injection in the retina of mice with Lenti-ONE EPI.

Furthermore, GEG Tech provides several types of Lenti-ONE EPI which have different features of expression to adjusting your project.

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