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Lenti-ONE GFP carries the transgene green fluorescent protein. Its expression can be assessed by fluorescent microscopy or by flow cytometry, thus giving valuable informations about the transduction efficiency of the vector.
ChR2 cDNA encodes for Channelrhodopsin-2 from Chlamydomonas reinhardtii (NCBI Reference Sequence: XP_001701725.1, 927bp). ChR2 is used in optogenetics for light activation of neural cells. It is a cation channel that, when activated by blue light, depolarizes the cell. In doing so, it gives rise to an action potential. To make its expression easily traceable, it is fused with the GFP cDNA encoding a green fluorescent protein (sequence from Aequorea victoria genome, AAB02574.1, 717bp). This fluorescent reporter protein allows the monitoring of expression and transduction by fluorescent microscopy or by flow cytometry.
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A few neuron specific promoters are available, such as NSE or Synapsin. They will allow neuron specific expression of the Channelrhodopsin.
