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This lentiviral vector carries the AOX cDNA encoding an alternative oxidase (sequence from Ciona intestinalis genome, DM193474.1, 1110 pb). This alternative oxidase is part of the electron transport chain in mitochondria. It contributes to the reduction of ROS production when overexpressed in mammalian cells.
AOX: Alternative Oxidase
Coenzyme Q: Ubiquinone
C: Cytochrome c
Complex I: NADH coenzyme Q reductase
Complex II: Succinate dehydrogenase
Complex III: cytochrome bc1 complex
Complex IV: Cytochrome c oxidase
Complex V: ATP synthase
In normal conditions, the AOX is inactive and thus doesn’t interfere with the normal electron flow from complex I to complex IV. However, when CIII and/or CIV are blocked, the electron flow is blocked and the Quinone pool becomes highly reduced which activates the AOX. The activation of the AOX in these situations allows:
Alternative oxidase expression in the mouse enables bypassing cytochrome c oxidase blockade and limits mitochondrial ROS overproduction.
More than 95% efficiency in mice with GEG Tech vectors
