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VECTOR GENOME SPECIFICATIONS

The vector genome is made of several elements. Thanks to the flexibility of our production system, every element can be customized according to your project. You can find below the specifications of all the promoters and transgenes available.

 

Promoter

Short Code
Full Name
Origin
Cell Specificity
AP2
Fragment enhancer + adipocyte P2 minimal
Mouse
Adipocytes
ARR3lg
Arrestin 3 long
Pig
Retina
ARR3sh
Arrestin 3 short
Pig
Retina
CAG
Cytomegalovirus early enhancer + chicken b-actin promoter
Synthetic
Ubiquitous
CAMKII
Calcium/calmodulin-dependent kinase II promoter
Mouse
Neurons
CMV
Cytomegalovirus promoter
Cytomegalovirus
Ubiquitous
EF1a
Elongation factor 1 alpha 1
Human
Various organs
FLT1
Fms-like tyrosine kinase-1
Human
Endothelial cells
GFAP
Glial fibrillary acidic protein
Human
Glial cells
NSE
Neuron specific enolase
Rat
Neurons
PGK
Phosphoglycerate kinase 1 promoter
Mouse
Ubiquitous
RHO
Rhodopsin
Mouse
Retina
SYN
Synapsin
Human
Neurons
UBI
Ubiquitin C
Human
Ubiquitous

 

shopping cart  See Promoter Set - Starter

 

Transgene

Short Code
Full Name
Origin
Optimization*
AOX
Alternative oxidase
Ciona intestinalis
Yes (mammalian)
BCL2
B-cell CLL/lymphoma 2
Human
 
BDNF
Brain-derived neurotrophic factor
Human
 
CNTF
Ciliary neurotrophic factor
Human
 
Cre-NLS
Cyclization recombinase fused with a nuclear localization signal
Enterobacterio phage P1
 
GDNF
Glial cell derived neurotrophic factor
Human
 
GFP
Enhanced green fluorescent protein
Aequorea victoria
 
GPx
Glutathione peroxidase 1
Human
 
GUCY2Dmut
Guanylate cyclase 2D
Human
 
hrGFPII
Humanized recombinant green fluorescent protein II
Renilla reniformis
Yes (mammalian)
LUC
Firefly luciferase PpyLuc1
Photinus pyralis
Yes (human)
MyoD
Myogenic differentiation
Mouse
Yes (mammalian)
NEO
Neomycin
Escherichia coli
 
PhiC31-NLS
Integrase phiC31 fused with a nuclear localization signal
Streptomyces phage phi C
 
SOD
Superoxide dismutase 1
Human
 
VEGF121
Vascular endothelial growth factor 121
Human
Yes (mammalian)
VEGF165a
Vascular endothelial growth factor 165a
Human
Yes (mammalian)
VEGF165b
Vascular endothelial growth factor 165b
Human
Yes (mammalian)

 

*Codon optimization: to maximize protein expression in particular species. Note that this optimization does not prevent expression in other species

 

PRE

PRE stands for Post transcriptional Regulatory Element. The one proposed by GEG Tech is from the Woodchuck hepatitis virus (WPRE). It's expected to improve the stability of the messenger RNA, thereby increasing the transgene expression level.

 

Backbone Options

Beside the choice of promoter and transgene, you can choose to add some backbone options to your vector genome. They have various functions:

  • The attB sequence corresponds to the attachment sequence from a bacterial genome recognized by the recombinase of phage phiC31. Lentiviral genomes containing this sequence may recombine with the target cell genome in attP sites or pseudo attP sites when the phage recombinase is present in the cell nucleus. For instance, this sequence is useful in a two-vector system where one vector carries attB along with the sequence of interest, and the other carries the recombinase.

    shopping cart  See vectors with attB or phiC31 vectors

  • The LTR(-) short code is for vector genomes containing substitutions in both 5' and 3' LTR preventing integrase recognition. Normally, LTRs contain attachment sequence located at both extremities of the lentiviral genome (5' and 3' LTRs), which are recognized by the integrase. This recognition is necessary for the integrase to catalyze the recombination of the lentiviral genome with the host cell chromosome. Thus, LTR(-) vectors have a highly reduced integration frequency, allowing stable transgene expression in non-dividing cells and transient expression in dividing cells.

    shopping cart  See LTR(-) vectors