The vector genome is made of several elements. Thanks to the flexibility of our production system, every element can be customized according to your project. You can find below the specifications of all the promoters and transgenes available.
PRE stands for Post transcriptional Regulatory Element. The one proposed by GEG Tech is from the Woodchuck hepatitis virus (WPRE). It's expected to improve the stability of the messenger RNA, thereby increasing the transgene expression level.
Beside the choice of promoter and transgene, you can choose to add some backbone options to your vector genome. They have various functions:
The attB sequence corresponds to the attachment sequence from a bacterial genome recognized by the recombinase of phage phiC31. Lentiviral genomes containing this sequence may recombine with the target cell genome in attP sites or pseudo attP sites when the phage recombinase is present in the cell nucleus. For instance, this sequence is useful in a two-vector system where one vector carries attB along with the sequence of interest, and the other carries the recombinase.
The LTR(-) short code is for vector genomes containing substitutions in both 5' and 3' LTR preventing integrase recognition. Normally, LTRs contain attachment sequence located at both extremities of the lentiviral genome (5' and 3' LTRs), which are recognized by the integrase. This recognition is necessary for the integrase to catalyze the recombination of the lentiviral genome with the host cell chromosome. Thus, LTR(-) vectors have a highly reduced integration frequency, allowing stable transgene expression in non-dividing cells and transient expression in dividing cells.
See LTR(-) vectors