GEG Tech offers three lines of vectors: Lenti-ONE™, Lenti-ONE™ Epi and Lenti-ONE™ Trans, each of them defined by the vector genome integration capability. The choice between these lines will determine the level and duration of your transgene expression.
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Lenti-ONE vectors are classic lentiviral vectors. Let’s see how they work.
Here is what a HIV-1 derived lentiviral vector particle looks like. It consists of several elements: two RNA molecules and proteins contained in a capside surrounded by an envelope.
The first event of the transduction process is the contact of the particle with cell membrane. The vector genome can then enter the cytoplasm. The RNA vector genome is then reverse-transcribed by the vector reverse-transcriptase (RT) into linear double stranded DNA. After translocation in the cell nucleus, the DNA is integrated into the cell chromatin by the vector integrase. Using the cell machinery, your protein of interest is synthetized from the integrated vector genome. With the integration of the vector genome, the protein is assured to have a high-level and long-term expression.
Lenti-ONE Epi vectors are non-integrative lentiviral vectors. Let's see how they work.
Beside integrating into the chromatin, the DNA of viral origin can also form circles which can be used as template for transcription. If we disable the vector integrase, the vector is no longer integrated but only circularized. Those circles are now the only template used for your protein synthesis. Due to the dilution effect of cell growth, these circles can provide short-term expression in dividing cells. However in non-dividing cells, you will have a long-term expression, yet lower than from integrated vectors.
If you want transient expression of your transgene even in non-dividing cells, Lenti-ONE Trans vectors are what you need. How does it work?
In a classic lentiviral vector, the reverse-transcriptase binds to a specific region of the vector genome called PBS. In our Lenti-ONE Trans vectors, the PBS region is deleted from the vector genome. The binding of RT and reverse-transcription are made impossible. And without reverse-transcription, the whole cycle is impaired. Your protein synthesis now relies on a very discreet process: translation directly from the primary RNA vector genome. Since RNAs have a short lifetime in the cytoplasm, the protein expression will be highly transient, even in non-dividing cells.
The three lines of GEG-tech vectors uniquely provide you with the opportunity to delimitate your transgene expression in time.