0

Shopping cart

0 items - $0.00

INTEGRASE

There are six integrases available for your vector: the wild-type HIV-1 integrase and five mutations of it. Three of them are non-integrative mutants and two are LEDGF-interaction mutants.

question mark  See FAQ on Integrases

shopping cart  See Integrase Set - Starter

 

logo GEG IN(WT) is the wild type HIV-1 integrase. This viral enzyme is responsible for the recombination of the vector genome with host cell chromatin. This way, the transgene has a robust and long-term expression in dividing and non-dividing cells.

question mark  See FAQ on Lenti-ONE™

shopping cart  See vectors with IN(WT) integrase

 

logo GEG IN(D64V) is the HIV-1 integrase with a substitution in the catalytic site (64 D>V). This viral enzyme is normally responsible for the recombination of the vector genome with host cell chromosome. This process is inactivated due to the mutation. Vectors produced with this integrase are deficient for integration. Transgene expression relies on the episomal forms of the vector genome.

question mark  See FAQ on Lenti-ONE™ Epi

shopping cart  See vectors with IN(D64V) integrase

 

logo GEG IN(LQ) is also a non-integrative mutation of the HIV-1 integrase, but with a substitution in the LQ basic region (186 K>Q, 214 Q>L, 216 Q>L). Vectors produced with this integrase are also deficient for integration and transgene expression relies on the episomal forms of the vector genome.

question mark  See FAQ on Lenti-ONE™ Epi

shopping cart  See vectors with IN(LQ) integrase

 

logo GEG IN(N) is the third non-integrative mutant of integrase, with a substitution in the N basic region (262 AAH>RRK). Vectors produced with this integrase are also deficient for integration and transgene expression relies on the episomal forms of the vector genome.

question mark  See FAQ on Lenti-ONE™ Epi

shopping cart  See vectors with IN(N) integrase

 

logo GEG The IN(R166A) integrase has a substitution in the codon 166 (166 R>A). Usually, the vector genome integration in the host cell chromatin is subject to a bias toward transcriptionally active regions due to an interaction between the integrase and LEDGF, a cellular factor. The substitution is expected to abolish IN–LEDGF interaction and to make the vector genome integration profile more random in the target cell genome.

question mark  See FAQ on Lenti-ONE™

shopping cart  See vectors with IN(R166A) integrase

 

logo GEG Similarly, the IN(Q168A) integrase contains a substitution (codon 168 Q>A) abolishing interactions between the viral integrase and LEDGF, making the vector genome integration profile more random in the target cell genome.

question mark  See FAQ on Lenti-ONE™

shopping cart  See vectors with IN(Q168A) integrase

 

REVERSE-TRANSCRIPTASE

There are three reverse transcriptases (RT) available for your vector: two with one mutation, D110E or R478Q and one with a double mutation D110E/E478Q.

question mark  See FAQ on Lenti-ONE Trans

shopping cart  See Reverse-Transcriptase Set - Starter

 

logo GEG RT(D110E): the HIV-1 reverse-transcriptase with one substitution (110 D>E). This viral enzyme is responsible for the reverse transcription of the vector RNA genome into DNA. This process is inactivated due to the mutation. Vectors produced with this reverse-transcriptase are deficient for the reverse-transcription step. The RNA vector genome is thus taken in charge by the host cell machinery as an mRNA and directly translated into a protein.

question mark  See FAQ on Lenti-ONE Trans

shopping cart  See vectors with RT(D110E) reverse-transcriptase

 

logo GEG RT(E478Q) is the also a reverse-transcriptase with one substitution (478 E>Q). Vectors produced with this reverse-transcriptase are also deficient for the reverse-transcription step.

question mark  See FAQ on Lenti-ONE Trans

shopping cart  See vectors with RT(E478Q) reverse-transcriptase

 

logo GEG RT(D110E/E478Q) is the third inactivated mutation of the HIV-1 reverse-transcriptase. It has two substitutions (110 D>E and 478 E>Q), making the reverse-transcription step impossible. The RNA vector genome is thus taken in charge by the host cell machinery as an mRNA and directly translated into a protein.

question mark  See FAQ on Lenti-ONE Trans

shopping cart  See vectors with RT(D110E/E478Q) reverse-transcriptase