The two envelopes available provide different cell specificities, affecting the transduction of your vector. So you must choose the one that will best fit your project purposes.
Modulation of cell-specificity expression through the choice of vector envelope
The very first step of transduction is the fixation of the viral particle to the target cell. For that to happen, there must be a recognition between the envelope glycoprotein of the vector, and receptors on the cell membrane. The existing receptors on the cell membrane vary according to multiple factors, including the cell type. Some receptors are ubiquitous and some are specific to a cell type. GEG-tech offers two types of lentiviral vectors pseudotyped with two different envelope glycoproteins: VSV and Mokola. The VSV-G glycoprotein binds with a membrane receptor present on most cell types. Therefore vectors with a VSV pseudotype are able to transduce a wide range of cell types. The mokola-G glycoprotein, on the contrary, binds to a receptor specific to glial cells. Only glial cells will be transduced, and your transgene will have a cell type-specific expression.
Let’s have a visual on real cells with sections of murine brain after stereotactical injection of PGK GFP lentiviral vectors. With VSV pseudotyped vectors, you can see that both neurons and glial cells express GFP. But with Mokola pseudotyped vectors, while glial cells widely express GFP, no expression can be seen in neurons.
Choosing the suitable envelope allows to achieve cell type-specific expression.
VSV is the envelope glycoprotein from the vesicular stomatitis virus. Lentiviral vectors produced with this envelope are expected to have a broad tropism and transduce a wide range of cell types.
Mokola is the envelope glycoprotein from the Lyssavirus Mokola. Lentiviral vectors produced with this envelope are expected to be glial cell specific in vivo